Publications
2024
Erythropoietin N-glycosylation of Therapeutic Formulations Quantified and Characterized: An Interlab Comparability Study of High-Throughput Methods
Róisín O’Flaherty, Manuela Amez Martín, Richard A. Gardner, Patrick M. Jennings, Pauline M. Rudd, Daniel I. R. Spencer and David Falck.
Biomolecules. 18 Jan 2024.
doi: 10.3390/biom14010125
2023
Elevated concentrations of Neu5Ac and Neu5,9Ac2 in human plasma: potential biomarkers of cardiovascular disease
Jack Cheeseman, Concepcion Badia, Georgia Elgood-Hunt, Richard A. Gardner, Duong N. Trinh, Marco P. Monopoli, Gunter Kuhnle, Daniel I.R. Spencer & Helen M.I. Osborn.
Glycoconjugate Journal. 22 Nov 2023.
doi: 10.1007/s10719-023-10138-3
Serum N-Glycomic Biomarkers Predict Treatment Escalation in Inflammatory Bowel Disease
Archana Shubhakar, Bas C Jansen, Alex T Adams, Karli R Reiding, Nicholas T Ventham, Rahul Kalla, Daniel Bergemalm, Paulina A Urbanowicz, Richard A Gardner, IBD-BIOM Consortium , Jonas Halfvarson, Jack Satsangi, Daryl L Fernandes, Daniel I R Spencer.
Journal of Crohn's and Colitis. 24 Jan 2023.
doi: 10.1093/ecco-jcc/jjad012 -- PMID: 36694402
2022
Generation and Characterization of Native and Sialic Acid-Deficient IgE
Alex J. McCraw, Richard A. Gardner, Anna M. Davies, Daniel I. R. Spencer, Melanie Grandits, Gerd K. Wagner ,James M. McDonnell, Sophia N. Karagiannis,Alicia Chenoweth, and Silvia Crescioli
International Journal of Molecular Sciences.
3 Nov 2022;23(21):13455.
doi: 10.3390/ijms232113455 PMID: 36362241
Free PMC article
Plant N-glycan breakdown by human gut Bacteroides
Lucy I. Crouch, Paulina A. Urbanowicz, Arnaud Baslé, Zhi-Peng Cai, Li Liu, Josef Voglmeir, Javier M Melo Diaz, Samuel T Benedict, Daniel I R Spencer, David N Bolam
Proc Natl Acad Sci USA. 27 Sep 2022;119(39):e2208168119.
doi: 10.1073/pnas.2208168119 Epub 19 Sep 2022. PMID: 36122227
Free PMC article
Biomolecular Corona Stability in Association with Plasma Cholesterol Level
Trinh DN, Radlinskaite M, Cheeseman J, Kuhnle G, Osborn HMI, Meleady P, Spencer DIR, Monopoli MP
Nanomaterials (Basel). 3 Aug 2022;12(15):2661
doi: 10.3390/nano12152661 PMID: 35957093
Free PMC article
Egg yolk sialylglycopeptide: purification, isolation and characterization of N-glycans from minor glycopeptide species
Javier Mauricio Melo Diaz, Alan B. Moran, Simon R. Peel, Jenifer L. Hendel, and Daniel I. R. Spencer
Organic and Biomolecular Chemistry Royal Society of Chemistry Journal. 16 May 2022.
doi: 10.1039/d2ob00615d
Probing the glycans accessibility in the nanoparticle biomolecular corona
Clemente E, Martinez-Moro M, Trinh DN, Soliman MG, Spencer DIR, Gardner RA, Kotsias M, Sánchez Iglesias A, Moya S, Monopoli MP
J Colloid Interface Sci. May 2022;613:563-574.
doi: 10.1016/j.jcis.2021.11.140 Epub 16 Dec 2022 -- PMID: 35066229
Structural and functional characterisation of a stable, broad-specificity multimeric sialidase from the oral pathogen Tannerella forsythia
Satur MJ, Urbanowicz PA, Spencer DIR, Rafferty J, Stafford GP
Biochem J.. 16 Sep 2022;479(17):1785-1806
doi: 10.1042/BCJ20220244 PMID: 35916484
Free PMC article
Extraction and purification of a High Mannose type oligosaccharide from Phaseolus lunatus beans by oxidative release with sodium hypochlorite
Melo Diaz JM, Peel SR, Spencer DIR, Hendel JL
Carbohydr Res. Jul 2022;517:108583
doi: 10.1016/j.carres.2022.108583 Epub May 2022. -- PMID: 35569240
Sialic Acid Derivatization of Fluorescently Labeled N-Glycans Allows Linkage Differentiation by Reversed-Phase Liquid Chromatography-Fluorescence Detection-Mass Spectrometry
Moran AB, Gardner RA, Wuhrer M, Lageveen-Kammeijer GSM, Spencer DIR
Anal Chem. 10 May 2022;;94(18):6639-6648.
doi: 10.1021/acs.analchem.1c02610 Epub 2022 Apr 28 -- PMID: 35482581
Free PMC article
Nanoparticle Biomolecular Corona-Based Enrichment of Plasma Glycoproteins for N-Glycan Profiling and Application in Biomarker Discovery
Trinh DN, Gardner RA, Franciosi AN, McCarthy C, Keane MP, Soliman MG, O'Donnell JS, Meleady P, Spencer DIR, Monopoli MP.
ACS Nano. 2022. 26 Apr 2022;16(4):5463-5475.
doi: 10.1021/acsnano.1c09564
Free PMC article
Development of an exoglycosidase plate-based assay for detecting α1-3,4 fucosylation biomarker in individuals with HNF1A-MODY
Demus D, Urbanowicz PA, Gardner RA, Wu H, Juszczak A, Štambuk T, Medvidović EP, Owen KR, Gornik O, Juge N, Spencer DIR
Glycobiology. 30 Mar 2022;32(3):230-238
doi: 10.1093/glycob/cwab107 -- PMID: 34939081
Free PMC article
Sialylation on O-linked glycans protects von Willebrand factor from macrophage galactose lectin-mediated clearance
DWard SE, O'Sullivan JM, Moran AB, Spencer DIR, Gardner RA, Sharma J, Fazavana J, Monopoli M, McKinnon TAJ, Chion A, Haberichter S, O'Donnell JS
Haematologica. 1 Mar 2022;;107(3):668-679
doi: 10.3324/haematol.2020.274720 -- PMID: 33763999 -- PMCID: PMC8883566
Free PMC article
Quantitative Standards of 4-O-Acetyl- and 9-O-Acetyl-N-Acetylneuraminic Acid for the Analysis of Plasma and Serum
Cheeseman J, Badia C, Thomson RI, Kuhnle G, Gardner RA, Spencer DIR, Osborn HMI
ChemBioChem. 4 Mar 2022;23(5):e202100662.
doi: 10.1002/cbic.202100662 Epub 18 Jan 2022 -- PMID: 34874597
Free PMC article
N-glycan Characterization by Liquid Chromatography Coupled with Fluorimetry and Mass Spectrometry
Gardner RA, Urbanowicz PA, Spencer DIR
Methods in Molecular Biology. 2022;2370:267-280.
doi: 10.1007/978-1-0716-1685-7_13 -- PMID: 34611874
2021
Glycoengineering of Therapeutic Antibodies with Small Molecule Inhibitors
Li S, McCraw AJ, Gardner RA, Spencer DIR, Karagiannis SN, Wagner GK
Antibodies. 4 Nov 2021.
doi: 10.3390/antib10040044 -- PMID: 34842612
Automation of Immunoglobulin Glycosylation Analysis
Hendel JL, Gardner RA, Spencer DIR
Antibody Glycosylation - Experientia Supplementum Book Series. 23 Oct 2021.
doi: 10.1007/978-3-030-76912-3_5 -- doi: 10.1007/978-3-030-76912-3_5 -- doi: 10.1007/978-3-030-76912-3_5 -- PMID: 34687010
Equal mixing time enables the optimization of IgG4 MAb production with minimal impact on glycosylation profiles
Wiegmann V, Gardner RA, Spencer DIR, Baganz F
Biotechnology Journal. Epub 21 Sep 2021.
doi:10.1002/biot.202100360
Expression and characterization of a novel single-chain anti-vascular endothelial growth factor antibody in the goat milk
Leiva MJ, Parra NC, Contreras MA, KCS Tavares, Macaya L, Martins L, Gaudencio S, Hidalgo A, Kozak RP, González A, Sánchez O, Toledo JR, Montesino R
Journal of Biotechnology. 10 Sep 2021.
doi: 10.1016/j.jbiotec.2021.06.025 -- PMID: 34224759
Sialic acid as a potential biomarker for cardiovascular disease, diabetes and cancer
Cheeseman J, Kuhnle G, Stafford G, Gardner RA, Spencer DIR, Osborn HM
Biomarkers in Medicine. Aug 2021.
doi: 10.2217/bmm-2020-0776 -- PMID: 34241547
A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS
Kotsias M, Madunić K, Nicolardi S, Kozak RP, Gardner RA, Jansen BC, Spencer DIR, Wuhrer M
Glycoconjugate Journal. 20 Jul 2021.
doi: 10.1007/s10719-021-10003-1 -- PMID: 34283362
Interlaboratory evaluation of plasma N-glycan antennary fucosylation as a clinical biomarker for HNF1A-MODY using liquid chromatography methods
Demus D, Jansen BC, Gardner RA, Urbanowicz PA, Wu H, Štambuk T, Juszczak A, Medvidović EP, Juge N, Gornik O, Owen KR, Spencer DIR
Glycoconjugate Journal. 25 Mar 2021.
doi: 10.1007/s10719-021-09992-w -- PMID: 33765222
Tsetse salivary glycoproteins are modified with paucimannosidic N-glycans, are recognised by C-type lectins and bind to trypanosomes
Kozak RP, Mondragon-Shem K, Williams C, Rose C, Perally S, Caljon G, Van Den Abbeele J, Wongtrakul-Kish K, Gardner RA, Spencer D, Lehane MJ, Acosta-Serrano A
PLOS Neglected Tropical Diseases. 2 Feb 2021.
doi: 10.1371/journal.pntd.0009071 -- PMID: 33529215
2020
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
Crouch LI, Liberato MV, Urbanowicz PA, Baslé A, Lamb CA, Stewart CJ, Cooke K, Doona M, Needham S, Brady RR, Berrington JE, Madunic K, Wuhrer M, Chater P, Pearson JP, Glowacki R, Martens EC, Zhang F, Linhardt RJ, Spencer DIR, Bolam DN
Nature Communications. 11 Aug 2020.
doi: 10.1038/s41467-020-17847-5 -- PMID: 32782292
Insights into the salivary N‑glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis
Mondragon-Shem K, Wongtrakul-Kish K, Kozak RP, Yan S, Wilson IBH, Paschinger K, Rogers ME, Spencer DIR, Acosta-Serrano A.
Scientific Reports. 31 Jul 2020.
doi:10.1038/s41598-020-69753-x
Fucosidases from the human gut symbiont Ruminococcus gnavus
Wu H, Rebello O, Crost EH, David Owen C, Walpole S, Bennati-Granier C, Ndeh D, Monaco S, Hicks T, Colvile A, Urbanowicz PA, Walsh MA, Angulo J, Spencer DIR, Juge N.
Cellular and Molecular Life Sciences. Apr 2020.
doi:10.1007/s00018-020-03514-x
A Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Assay for the Relative Quantitation of Antennary Fucosylated N-Glycans in Human Plasma
Rebello OD, Nicolardi S, Lageveen-Kammeijer GSM, Nouta J, Gardner RA, Mesker WE, Tollenaar RAEM, Spencer DIR, Wuhrer M, Falck D.
Frontiers in Chemistry. 28 Feb 2020.
doi: 10.3389/fchem.2020.00138
2019
Characterization of Porphyromonas gingivalis sialidase and disruption of its role in host-pathogen interactions
Frey AM, Satur MJ, Phansopa C, Honma K, Urbanowicz PA, Spencer DIR, Pratten J, Bradshaw D, Sharma A, Stafford G.
Microbiology. Nov 2019.
doi: 10.1099/mic.0.000851
NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods
De Leoz MLA et al.
Molecular & Cellular Proteomics. October 30, 2019, mcp.RA119.001677;
doi: 10.1074/mcp.RA119.001677
Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis
Kotsias M, Blanas A, van Vliet SJ, Pirro M, Spencer DIR, Kozak RP.
PLoS One. 2019 Oct 7.
doi: 10.1371/journal.pone.0223270
Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci
Briliūtė J, Urbanowicz PA, Luis AS, Baslé A, Paterson N, Rebello O, Hendel J, Ndeh DA, Lowe EC, Martens EC, Spencer DIR, Bolam DN, Crouch LI.
Nature Microbiology. 2019 Jun 3.
doi: 10.1038/s41564-019-0466-x
Current Stage of Commercially Available Glycans to Support Realization of Biologic Drugs
Hendel JL, Fernandes DL.
Synthetic Glycomes. Chapter 17. P393-420. Edited by Guan W, Li L and Wang PG, London: The Royal Society of Chemistry 2019.
doi:10.1039/9781788016575-00393
Improved and semi-automated reductive β-elimination workflow for higher throughput protein O-glycosylation analysis.
Kotsias M, Kozak RP, Gardner RA, Wuhrer M, Spencer DIR.
PLoS One. 2019;14(1):e0210759. Published 2019 Jan 17.
doi:10.1371/journal.pone.0210759
2018
Transcriptional activation of fucosyltransferase (FUT) genes using the CRISPR-dCas9-VPR technology reveals potent N-glycome alterations in colorectal cancer cells.
Blanas A, Cornelissen LAM, Kotsias M, van der Horst JC, van de Vrugt HJ, Kalay H, Spencer DIR, Kozak RP, van Vliet SJ.
Glycobiology. 2018 Nov 22.
doi: 10.1093/glycob/cwy096
High-throughput serum N-glycomics: method comparison and application to study rheumatoid arthritis and pregnancy-associated changes.
Reiding KR, Bondt A, Hennig R, Gardner RA, O'Flaherty R, Trbojevic-Akmacic I, Shubhakar A, Hazes J, Reichl U, Fernandes DL, Pucic-Bakovic M, Rapp E, Spencer DIR, Dolhain R, Rudd P, Lauc G, Wuhrer M.
Molecular & Cellular Proteomics. 2018 Sep 21. pii: mcp.RA117.000454.
doi: 10.1074/mcp.RA117.000454
GlycoStore: a database of retention properties for glycan analysis.
Zhao S, Walsh I, Abrahams JL, Royle L, Nguyen-Khuong T, Spencer D, Fernandes DL, Packer NH, Rudd PM, Campbell MP.
Bioinformatics. 2018 Sep 15;34(18):3231-3232.
doi: 10.1093/bioinformatics/bty319
HappyTools: A software for high-throughput HPLC data processing and quantitation.
Jansen BC, Hafkenscheid L, Bondt A, Gardner RA, Hendel JL, Wuhrer M, Spencer DIR.
PLoS One. 2018 Jul 6;13(7):e0200280.
doi: 10.1371/journal.pone.0200280
Towards automation of glycomic profiling of complex biological materials.
Shubhakar A, Pang PC, Fernandes DL, Dell A, Spencer DIR, Haslam SM.
Glycoconjugate Journal. 2018 Jun 16.
doi: 10.1007/s10719-018-9825-8
Engineering and stable production of recombinant IgE for cancer immunotherapy and AllergoOncology.
Crescioli S, Chiaruttini G, Mele S, Ilieva KM, Pellizzari G, Spencer DIR, Gardner RA, Lacy KE, Spicer JF, Tutt ANJ, Wagner GK, Karagiannis SN.
Journal of Allergy and Clinical Immunology. 2018 Apr;141(4):1519-1523.e9.
doi: 10.1016/j.jaci.2017.12.986
2017
Analysis of Three Epoetin Alpha Products by LC and LC-MS Indicates Differences in Glycosylation Critical Quality Attributes, Including Sialic Acid Content.
Thomson RI, Gardner RA, Strohfeldt K, Fernandes DL, Stafford GP, Spencer DIR, Osborn HMI.
Analytical Chemistry. 2017 Jun 20;89(12):6455-6462.
doi: 10.1021/acs.analchem.7b00353
2016
Variation of Human Salivary O-Glycome.
Kozak RP, Urbanowicz PA, Punyadeera C, Reiding KR, Jansen BC, Royle L, Spencer DI, Fernandes DL, Wuhrer M.
PLoS One. 2016 Sep 9;11(9):e0162824.
doi: 10.1371/journal.pone.0162824
Automated High-Throughput Permethylation for Glycosylation Analysis of Biologics Using MALDI-TOF-MS.
Shubhakar A, Kozak RP, Reiding KR, Royle L, Spencer DI, Fernandes DL, Wuhrer M.
Analytical Chemistry. 2016 Sep 6;88(17):8562-9.
doi: 10.1021/acs.analchem.6b01639
Composition and Antigenic Effects of Individual Glycan Sites of a Trimeric HIV-1 Envelope Glycoprotein.
Behrens AJ, Vasiljevic S, Pritchard LK, Harvey DJ, Andev RS, Krumm SA, Struwe WB, Cupo A, Kumar A, Zitzmann N, Seabright GE, Kramer HB, Spencer DI, Royle L, Lee JH, Klasse PJ, Burton DR, Wilson IA, Ward AB, Sanders RW, Moore JP, Doores KJ, Crispin M.
Cell Reports. 2016 Mar 22;14(11):2695-706.
doi: 10.1016/j.celrep.2016.02.058
Longitudinal monitoring of immunoglobulin A glycosylation during pregnancy by simultaneous MALDI-FTICR-MS analysis of N- and O-glycopeptides.
Bondt A, Nicolardi S, Jansen BC, Stavenhagen K, Blank D, Kammeijer GS, Kozak RP, Fernandes DL, Hensbergen PJ, Hazes JM, van der Burgt YE, Dolhain RJ, Wuhrer M.
Scientific Reports. 2016 Jun 15;6:27955.
doi: 10.1038/srep27955
2015
Characterization of a sialate-O-acetylesterase (NanS) from the oral pathogen Tannerella forsythia that enhances sialic acid release by NanH, its cognate sialidase.
Phansopa C, Kozak RP, Liew LP, Frey AM, Farmilo T, Parker JL, Kelly DJ, Emery RJ, Thomson RI, Royle L, Gardner RA, Spencer DI, Stafford GP.
Biochemical Journal. 2015 Dec 1;472(2):157-67.
doi: 10.1042/BJ20150388
Parasite Glycobiology: A Bittersweet Symphony.
Rodrigues JA, Acosta-Serrano A, Aebi M, Ferguson MA, Routier FH, Schiller I, Soares S, Spencer D, Titz A, Wilson IB, Izquierdo L.
PLoS Pathogens. 2015 Nov 12;11(11):e1005169.
doi: 10.1371/journal.ppat.1005169
Comparison of procainamide and 2-aminobenzamide labeling for profiling and identification of glycans by liquid chromatography with fluorescence detection coupled to electrospray ionization-mass spectrometry.
Kozak RP, Badía Tortosa C, Fernandes DL, Spencer DI.
Analytical Biochemistry. 2015 Oct 1;486:38-40.
doi: 10.1016/j.ab.2015.06.006
Glycan Microheterogeneity at the PGT135 Antibody Recognition Site on HIV-1 gp120 Reveals a Molecular Mechanism for Neutralization Resistance.
Pritchard LK, Spencer DI, Royle L, Vasiljevic S, Krumm SA, Doores KJ, Crispin M.
Journal of Virology. 2015 Jul;89(13):6952-9.
doi: 10.1128/JVI.00230-15
Glycan clustering stabilizes the mannose patch of HIV-1 and preserves vulnerability to broadly neutralizing antibodies.
Pritchard LK, Spencer DI, Royle L, Bonomelli C, Seabright GE, Behrens AJ, Kulp DW, Menis S, Krumm SA, Dunlop DC, Crispin DJ, Bowden TA, Scanlan CN, Ward AB, Schief WR, Doores KJ, Crispin M.
Nature Communications. 2015 Jun 24;6:7479.
doi: 10.1038/ncomms8479
Development of a Schistosoma mansoni shotgun O-glycan microarray and application to the discovery of new antigenic schistosome glycan motifs.
van Diepen A, van der Plas AJ, Kozak RP, Royle L, Dunne DW, Hokke CH.
International Journal for Parasitology. 2015 Jun;45(7):465-75.
doi: 10.1016/j.ijpara.2015.02.008
Changes to serum sample tube and processing methodology does not cause Intra-Individual [corrected] variation in automated whole serum N-glycan profiling in health and disease.
Ventham NT, Gardner RA, Kennedy NA, Shubhakar A, Kalla R, Nimmo ER; IBD-BIOM Consortium, Fernandes DL, Satsangi J, Spencer DI.
PLoS One. 2015 Apr 1;10(4):e0123028.
doi: 10.1371/journal.pone.0123028
High-Throughput Analysis and Automation for Glycomics Studies.
Shubhakar A, Reiding KR, Gardner RA, Spencer DI, Fernandes DL, Wuhrer M.
Chromatographia. 2015;78(5-6):321-333.
doi: 10.1007/s10337-014-2803-9
Mass spectrometry for glycosylation analysis of biopharmaceuticals.
Dotz V, Haselberg R, Shubhakar A, Kozak RP, Falck D, Rombouts Y, Reusch D, Somsen GW, Fernandes DL, Wuhrer M.
TrAC Trends in Analytical Chemistry. Volume 73, November 2015, Pages 1-9.
doi: 10.1016/j.trac.2015.04.024
2014
The role of glycosylation in IBD.
Theodoratou E, Campbell H, Ventham NT, Kolarich D, Pučić-Baković M, Zoldoš V, Fernandes D, Pemberton IK, Rudan I, Kennedy NA, Wuhrer M, Nimmo E, Annese V, McGovern DP, Satsangi J, Lauc G.
Nature Reviews Gastroenterology & Hepatology. 2014 Oct;11(10):588-600.
doi: 10.1038/nrgastro.2014.78
Carbohydrate sequence of the prostate cancer-associated antigen F77 assigned by a mucin O-glycome designer array.
Gao C, Liu Y, Zhang H, Zhang Y, Fukuda MN, Palma AS, Kozak RP, Childs RA, Nonaka M, Li Z, Siegel DL, Hanfland P, Peehl DM, Chai W, Greene MI, Feizi T.
Journal of Biological Chemistry. 2014 Jun 6;289(23):16462-77.
doi: 10.1074/jbc.M114.558932
Improved nonreductive O-glycan release by hydrazinolysis with ethylenediaminetetraacetic acid addition.
Kozak RP, Royle L, Gardner RA, Bondt A, Fernandes DL, Wuhrer M.
Analytical Biochemistry. 2014 May 15;453:29-37.
doi: 10.1016/j.ab.2014.02.030
Mutations in B4GALNT1 (GM2 synthase) underlie a new disorder of ganglioside biosynthesis.
Harlalka GV, Lehman A, Chioza B, Baple EL, Maroofian R, Cross H, Sreekantan-Nair A, Priestman DA, Al-Turki S, McEntagart ME, Proukakis C, Royle L, Kozak RP, Bastaki L, Patton M, Wagner K, Coblentz R, Price J, Mezei M, Schlade-Bartusiak K, Platt FM, Hurles ME, Crosby AH.
Brain: A Journal of Neurology. 2013 Dec;136(Pt 12):3618-24.
doi: 10.1093/brain/awt270
2013
Effect of a tail piece cysteine deletion on biochemical and functional properties of an epidermal growth factor receptor-directed IgA2m(1) antibody.
Brunke C, Lohse S, Derer S, Peipp M, Boross P, Kellner C, Beyer T, Dechant M, Royle L, Liew LP, Leusen JH, Valerius T.
mAbs. 2013 Nov-Dec;5(6):936-45.
doi: 10.4161/mabs.26396
2012
Suppression of peeling during the release of O-glycans by hydrazinolysis.
Kozak RP, Royle L, Gardner RA, Fernandes DL, Wuhrer M.
Analytical Biochemistry. 2012 Apr 1;423(1):119-28.
doi: 10.1016/j.ab.2012.01.002
Protein O-glycosylation analysis.
Zauner G, Kozak RP, Gardner RA, Fernandes DL, Deelder AM, Wuhrer M.
Biological Chemistry. 2012 Aug;393(8):687-708.
doi: 10.1515/hsz-2012-0144