Exoglycosidases:

- Cleave specific terminal-monosacharides from glycans
- Allows detailed structural characterisation of glycans (monosaccharide type, linkage and sequence)
- All enzymes are tested for the absence of proteolytic or unexpected glycosidic activity

Example Enzyme Sequencing:


Ludger Enzyme Sequencing Example

Workflow for exoglycosidase sequencing of glycans based on HPLC/LC-MS detection:

Ludger Exoglycosidase Sequencing Workflow

click to enlarge 

Enzymatic or chemical release of glycans
See Ludger endoglycosidase and chemical glycan release pages for product information

Fluorescent labelling and clean-up
See Ludger glycan labelling page for information on 2-AB, 2-AA, procainamide and Vtag labelling kits
See Ludger glycan clean-up page for product information

Post-glycosidase clean-up
See Ludger glycan clean-up page for information on LC-EXO-A6 and LC-EXO-96


Exoglycosidases Products:

Sialidase Au α(2-3,6,8,9)

Ludger Sialidase Au enzyme structure

E-S001

α(2-3,6,8,9) Sialidase cleaves all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. In addition, the enzyme will cleave branched sialic acids (linked to an internal residue).

Recombinant from Arthrobacter ureafaciens in E. coli

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-S001
  • Amount of Enzyme
  • 0.3 U / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


Sialidase Cp α(2-3,6)

Ludger Sialidase Cp enzyme structure

E-S005

Sialidase Cp cleaves all non-reducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins.

Recombinant from Clostridium perfringens in E. coli

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-S005
  • Amount of Enzyme
  • 0.9 U / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


Sialidase Sp α(2-3)

Ludger Sialidase Sp enzyme structure

E-S007

Sialidase Sp cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins.

Recombinant from Streptococcus pneumoniae in E. Coli

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-S007
  • Amount of Enzyme
  • 0.3 U / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


LudgerZyme Acetyl esterase (sialate-O-acetylesterase) kit

Ludger Acetyl Esterase enzyme structure

LZ-ACASE-KIT

To remove 9-, 8- and 7-O-acetyl groups from released sialic acids, released glycans or glycoproteins. Used for characterisation of highly sialylated biotherapeutics such as EPO, FSH and blood clotting factors.

Recombinant from Tannerella forsythia in E. Coli.

Product guide

Certificate of stability

  • Part Number
  • LZ-ACASE-KIT
  • Amount of Enzyme
  • 50 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 50 samples.


β(1-4)-galactosidase

Ludger Beta-(1-4)-galactosidase enzyme structure

E-BG07

Non-reducing terminal β(1-4)-Galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.

Recombinant from Streptococcus pneumoniae in E. coli

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-BG07
  • Amount of Enzyme
  • 0.18 U / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


β(1-3,4,6)-galactosidase

Ludger Beta-(1-3,4,6)-galactosidase enzyme structure

E-BG02

Cleaves all β1-3 and β1-4 linked non-reducing, terminal galactose. β1-6 linked galactose is released at a slower rate.

Bovine testes

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-BG02
  • Amount of Enzyme
  • 0.5 U / 200 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 200 reactions.


α(1-3,6)-galactosidase

Ludger Alpha-(1-3,6)-galactosidase enzyme structure

E-AG02    *formerly E-AG01

Alpha Galactosidase from E. coli cleaves α(1-3) and α(1-6) linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.

Recombinant from E. coli in E. coli

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-AG02
  • Amount of Enzyme
  • 0.4 U / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


β(1-2,3,4,6)-N-acetylglucosaminidase

Ludger N-acetylglucosaminidase enzyme structure

E-GL01

N-acetylglucosaminidase cleaves all non-reducing terminal β-linked N-acetylglucosamine residues from complex carbohydrates and glycoproteins.

Recombinant from Streptococcus pneumoniae in E. coli

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-GL01
  • Amount of Enzyme
  • 2.4 U / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


α(1-2,3,6)-mannosidase

Ludger Alpha-(1-2,3,6)-mannosidase enzyme structure

E-AM01

Cleaves all α(1-2,3,6) linked mannose

From Jack Bean

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-AM01
  • Amount of Enzyme
  • 0.6 U / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


α(1,6) core mannosidase

Ludger Alpha-(1,6) core mannosidase enzyme structure

E-AM02

α(1-6) core mannosidase cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides.

Recombinant from Xanthamonas manihotis in E. Coli

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-AM02
  • Amount of Enzyme
  • 0.6 U / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


LudgerZyme α(1-3,4) Fucosidase Kit  *New Product

LudgerZyme Alpha-(1-3,4) Fucosidase Kit enzyme structure

LZ-FUCOSIDASE-01-KIT

The enzyme recognises α1-3,4 fucosylated glycans (e.g. Lewis X/A epitopes, including their sialylated counterparts) and hydrolyses terminal α1-3 and α1-4 fucosyl linkages in these substrates without the need to remove sialic acid.

Product guide
Certificate of stability

  • Part Number
  • LZ-FUCOSIDASE-01-KIT
  • Amount of Enzyme
  • 50 µL
  • Non-sialidase dependant hydrolysis of antennary fucose moieties
  • Effective on both glycopeptides and free glycans
  • Highly specific (α1-3,4 fucosylated glycans)
  • Kit includes enzyme plus reaction buffer.
  • Sufficient for up to 50 samples.

α(1-3,4)-fucosidase

Ludger Alpha-(1-3,4)-fucosidase enzyme structure

E-F134

α(1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures.

Xanthamonas manihotis

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-F134
  • Amount of Enzyme
  • 30 mU / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.